The gender neutral term for “ladies and gentleman” is “distinguished guests”. This has been around since at least the 60s, so no one is going to question your use of it.
Please, please use this along with “ladies and gentlemen” if you aren’t entirely sure of the gender of your audience.
hint: you can probably never be sure of the gender of your audience
one time when my dad was in college these guys found a carpet on the sidewalk and they needed furniture for their dorm so they were like “hell yeah free carpet” and they carried the carpet upstairs and it was really wet and heavy so they unrolled it to let it dry and a dead body fell out
I’M NOT SHITTING YOU
when kids stare at you for a long time